منابع مشابه
Depth - of - Focus in Microscopy
Digital image processing and analysis provide a powerful tool for describing optical systems. In this paper we show that conventional formulas for the depth-of-focus (∆z) of a high numerical aperture, diffraction-limited optical system are incorrect. Using standard diffraction wave theory we have (rediscovered d a formula for ∆z and then tested that formula under controlled experimental conditi...
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The capacity of proteins to function relies on a balance between molecular stability to maintain their folded state and structural flexibility allowing conformational changes related to biological function. Among many others, four different examples can be chosen. The giant protein titin is stretched and can unfold during muscle contraction providing passive elasticity to muscle tissue; myoglob...
متن کاملExtended focus depth for Fourier domain optical coherence microscopy.
We report on a new detection scheme for Fourier domain optical coherence microscopy that exhibits high transverse resolution along an axially extended focal range. Nearly constant transverse resolution of approximately 1.5 microm along a focal range of 200 microm is experimentally verified with a maximum sensitivity of 105 dB. A broad-bandwidth Ti:sapphire laser allowed for an axial resolution ...
متن کاملPixel Based Z-focus Control in Scanning Microscopy
Optical microscopes, arguably the most commonly used tools to characterize the building blocks of nature, are optimized for acquiring two-dimensional information. In particular, they are designed to capture images from the plane perpendicular to the optical axis, namely the XY plane. This represents a strong limitation, because the inherent 3D properties of samples require truly volumetric char...
متن کاملExtended depth-of-focus by digital holographic microscopy.
A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of moving the sample. Extended depth of focus of amplitude images has been demonstrated, but it has marginal interest for the metrological applic...
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ژورنال
عنوان ژورنال: Physics Today
سال: 2000
ISSN: 0031-9228,1945-0699
DOI: 10.1063/1.2405464